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In some cases, negative control experiments were performed using a polypeptide derived from residues 143-284 of chicken muscle α-tropomyosin [60].
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In such cases, negative controls may be used to identify the unwanted variation and separate it from the wanted variation.
The chemical composition of CSF varies during onset of meningitis, neurodegenerative disorders (positive controls) and in traumatic cases (negative controls).
In all cases, negative controls were performed by repeating the procedure and omitting the primary antibody.
In all cases, negative controls included substitution of the primary antibodies by PBS, or by non-relevant isotype controls.
Negative controls were omission of the primary antibody, goat nonimmune IgG, rabbit nonimmune IgG, rat nonimmune IgG or secondary antibody only; in all cases, negative controls showed insignificant staining.
For each case, negative control slides consisted of sections incubated with antibody vehicle or no immune rabbit or mouse serum.
A 5 base pair (bp) mismatch (indicated in lower case) negative control morpholino (5′-TCAcCATCTcTAcCCATCcTCcCT-3′) and a GeneTools standard control (SC) morpholino (to mutated human beta-globin gene; 5′-CCTCTTACCTCAGTTACAATTTATA-3′) were used.
For each case, negative controls were performed on serial sections.
In each case, negative controls were included in which the primary antibody was excluded.
In each case, negative controls were performed by substituting the primary antibodies by irrelevant IgG antibodies.
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