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In all cases, microvessel density (MVD) was evaluated by counting CD34-reactive endothelial cells or endothelial cell clusters.
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We also studied a series of 86 human breast carcinomas and demonstrated a significant association between TGFα and angiogenic index: TGFα + cases, 48.1± 28.0; TGFα- cases, 31.8± 20.0 microvessel density (P = 0.01).
We did not evaluate angiogenesis because our 110 tumour samples were core needle biopsies, and in most cases the microvessel density could not be reliably assessed on a core needle biopsy specimen [ 12].
Frequency of positive cases and intratumoral microvessel density (IMD) was compared among the group of lesions.
HIF2α protein was less frequently expressed in cases with a medium microvessel density (MVD); a high rate of expression was noted in cases with both low and high MVD (P = 0.006).
66 Our immunohistochemical analysis revealed that 34% of GC cases were positive for tetraspanin 8, and microvessel density was higher in tetraspanin 8-positive GC cases compared with tetraspanin 8-negative GC cases.
Hence, the large difference in intravasation score observed in these different cases is not explained by differences in microvessel density alone.
To access the sensitivities to the preexisting vascular network, we changed the microvessel density (MVD) to values of 0.5 time (Case a) and 1.5 time (Case c) of that in the baseline case (Case b).
Microvessel density alone is also a valuable tool in identifying potential progression to SCC in some cases of VIN III (Bamberger and Perrett, 2002).
Consistently the microvessel density (MVD), evaluated by CD34 staining, was high, with a significant higher MVD value in case 2 compared to case 1 (p = 0.037, t Student test).
Finally, both the microvessel density (MVD) and proliferation-related parameters (proliferation index, (PI) and apoptotic index (AI)) were evaluated in both RB cases.
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