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Recently, it has been shown that CTIP2 is expressed early during mouse development as well as in the adult animal, and in both cases, expression is most predominant in the skin/epithelial structures, CNS and thymus [19], [20].
In all six cases, expression is reduced in spt, suggesting that they are downstream targets of tbx16.
Knowledge on the regulation of expansin genes is still scarce, but in many cases expression is regulated by plant hormones (e.g. auxins) or abiotic stress (e.g. Bray 2004; Cosgrove 2005; Lee et al. 2005).
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In rare cases, expression was seen outside the pharynx: the pqn-57 reporter was also expressed near the rectum (supplementary material Fig. S3) and RNA in situ analysis showed rare abu-14 staining in the intestine (http://nematode.lab.nig.ac.jp).lab.nig.ac.jp
In all cases expression was lower in the co-treated mice.
In all four cases, expression was inferred from the microarray data but could not be confirmed by real-time PCR.
In all cases, expression was obtained in at least 25% of the injected embryos (see Table 3).
In some cases, expression was not detected by in situ hybridization in mice but may still be present at low levels.
In all cases, expression was corrected with that of β-actin measured on the same sample in parallel on the same plate.
In both cases expression was very similar to that of the endogenous AgG12, in terms of tissue- and sex-specificity and temporal profile in response to bloodmeal, with a clear peak at 24 h PBM (Figure 3b).
In the remaining cases expression was most intense on the luminal surface of tumours.
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