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Moreover, in both cases, cultures are time consuming and require direct sample handling, representing a risk of infection for laboratory personnel.
In all cases, cultures were maintained in the presence of saturating concentrations of exogenous EGF in order to limit any confounding effects from autocrine/juxtacrine EGFR activation [13].
In all cases, cultures were counterstained using Mayer's haematoxylin.
In some cases cultures were also treated for 24 h with 500 nM salubrinal, an inhibitor of endoplasmic reticulum stress.
In all cases, cultures were initiated from equal proportions of R → A ← L, G → L ← A, and C ← L populations.
In all cases, cultures were propagated and maintained by monthly sub-culturing, in which a small fragment of thallus tissue (~5-7 mm in diameter) was cut and placed on fresh medium.
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169 patients also had replicate blood sampling; PCR/ESI-MS was concordant in 85% of cases, culture in 55%.
In all cases, culture medium was supplemented with 100 units/ml penicillin and 100 µg/ml streptomycin.
In turn, cases with positive cultures must be a subset of all cases (culture positive, negative, or not done).
In these cases, culture supported by histological examination is used to confirm or rule out infection with M. bovis.
In all cases, culture medium consisted of X-VIVO 15 (Lonza, Walkersville, MD) supplemented with penicillin (100U/ml) and streptomycin (0.1mg/ml).
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