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In all cases, cell viability was assessed 24 h after the treatment.
In all cases, cell viability was over 90% as measured by trypan blue exclusion.
In all cases cell viability after drug treatment was greater than 98%.
In some cases, cell viability in monocultures was determined by measuring cell metabolic activity using Alamar Blue, a tetrazolium dye that is reduced by living cells to a colored product as previously described.
Similar(55)
Moreover, we can conclude that serum withdrawal for 48 hours rather decrease EBF adaptation and enhance cell detachment than the 24 hour FBS depletion and in the latter case cell viability was though not altered, thus, EBF cultured in 10% FBS represent a good model allowing studying the response to drugs that influence cell proliferation and pathways of airway remodeling in airway diseases.
In all cases, the cell viability increased even at high ethanol concentration (12% v/v) in the fruit pulp-supplemented medium compared with the control medium.
This approach can be used in cases where cell viability can be supported with an episome.
In both cases, when cell viability reached ∼50% of control cells, the caspase activation window could be detected.
In this case, the cell viability at the maximum of concentration (80 μmol L−1), slightly lower than the control, was caused by the natural light during the execution of experiments.
In each case, the cell viability was checked by the Trypan blue exclusion method and found to be >95%.
The calibrated model is then tested under three hypothetical intervention scenarios and in all cases predicts increased cell viability that agrees with experimental evidence.
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