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In all cases, cell fluorescence was analyzed in background-substracted images using commercially available software (VoxCell Scan, Visitech).
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In some cases cell nuclei were also identified for fluorescence imaging by incubation with 1 μM 4′,6-diamidino-2-phenylindole after antibody removal.
In all cases, cell responses were determined with a fluorescence microscope (Zeiss Axiovert 405 M) equipped with a 100 W mercury lamp and a Zeiss 02 filter combination (365/420 nm) for excitation and emission.
(b) Statistical analysis showing results of cell fluorescence for DHE.
corrected total cell fluorescence.
Cell fluorescence was analyzed at 488-nm excitation and applied to standard fluorescence compensation.
Cell fluorescence was measured by flow cytometry.
Fluorescence intensity was expressed as Corrected Total Cell Fluorescence (CTCF).
In all cases, cells retained morphological integrity and cell surface fluorescence, with consistently higher labeling for BCN than for DIBO, as detected by confocal microscopy and flow cytometry.
We also carry out single-cell fluorescence imaging studies on mammalian cell lines.
In certain cases, interphase fluorescence in situ hybridisation (I-FISH) can be used to decide on the tumour cell number.
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