The resulting transmission data are summarized in table 1, together with those from sCJD cases assayed for comparison.
Among the 68 cases assayed by FISH on both primary and paired metastatic site, 10 cases were amplified in both specimens (Table 2).
In both cases assayed, for syd-9 and nurf-1, the alternative reading frame runs through the final exon, is based on a single EST, and could simply reflect a rare splicing error.
To reduce the number of false positive cases, assay systems with higher specificity are needed.
In most cases, assay-related protocols are developed on an individual laboratory basis, resulting in a large number of different protocols being applied worldwide.
In five cases, specific assays could be designed with at least one primer in the element.
Then, the highly motile sperm population was adjusted to 7×106 cells/ml in HAM F-10 supplemented with 1% HSA (to support capacitation) or without HSA (in the case assays were performed with non-capacitated cells), and then incubated at 37°C in 5% CO2 on air for 4 h [2].
In the HPFS, cases were assayed in four separate analytical runs (cases up to January 1996, February 1996-January 1998, February 1998-January 2000, and February 2000 January 2004) [21], [22].
The PHS cases were assayed in two analytic runs [23], [24].
Protein content in both cases was assayed with the Bio-Rad protein reagent (Bio-Rad, Alcobendas, Spain).
