The resulting transmission data are summarized in table 1, together with those from sCJD cases assayed for comparison.
In most cases, assay-related protocols are developed on an individual laboratory basis, resulting in a large number of different protocols being applied worldwide.
In most cases, assays (not sampling) were done after the illness to determine its etiology.
Among the 68 cases assayed by FISH on both primary and paired metastatic site, 10 cases were amplified in both specimens (Table 2).
In both cases assayed, for syd-9 and nurf-1, the alternative reading frame runs through the final exon, is based on a single EST, and could simply reflect a rare splicing error.
In our case, assay for anti-IFN antibodies was not available at the onset of the disease in our patient, but the above antibodies were found to be positive at the age of 9 years.
Then, the highly motile sperm population was adjusted to 7×106 cells/ml in HAM F-10 supplemented with 1% HSA (to support capacitation) or without HSA (in the case assays were performed with non-capacitated cells), and then incubated at 37°C in 5% CO2 on air for 4 h [2].
Regarding the KASPar assays, in one case no SNP was detected and in four cases the assay did not work properly.
Although IFA can be used to confirm infection in these ambiguous cases, this assay is not widely used.
Five cross-reactivity signals had Rm/Rwt>0.7, but in these cases, the assay for the genuine mutation had Rm/Rwt>1.0.
