Sentence examples for casein zymography from inspiring English sources

We then used substrates and inhibitors for KLK5, KLK7, and KLK14 to explore the activity of each protease in the stratum corneum using casein zymography and an ex vivo desquamation assay.

Fig. 4 Casein zymography of proteases from K. rhizophila p3-3.

This AprX protease is solely responsible for casein hydrolysis as evidenced by casein zymography (Marchand et al. 2009b).

To investigate the EAAC in cancer metastasis, SK-Hep1 cells were treated with EAAC at various concentrations and then subjected to gelatin zymography, casein zymography and western blot to study the impacts of EAAC on metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-1/2 (TIMP-1/2), respectively.

Casein zymography was performed as described previously [31].

Gelatin or casein zymography was performed as described previously [10].

Production of MMPs and uPA by cancer or endothelial cells were analyzed by gelatin and plasminogen-casein zymography, respectively.

β-casein zymography is useful for detection of MMP-1, which appears as a doublet in the 54-kD region of the gel.

Before use, each pooled preparation of culture fluid was assessed for total and active MMP-1 by β-casein zymography and for total and active MMP-2 and MMP-9 by gelatin zymography.

The MMP-1 preparation appeared as a doublet at 52 and 57 kDa in β-casein zymography, and it was reactive with a rabbit polyclonal anti-MMP-1 antibody (AB806; Chemicon International, Temecula, CA, USA) by western blotting.

Protease activity was also analyzed by native-PAGE casein-zymography.