Sentence examples for casein buffer from inspiring English sources

Briefly, plates were coated overnight at 4°C with the FMP2.1 recombinant AMA1 antigen (100 µL/well, 0.5 µg/mL), after which they were blocked with a 0.5% boiled casein buffer for 1 hour at 22°C.

Non-specific binding was blocked using 10%% casein buffer.

The beads were washed three times with 100 ml casein buffer on a vacuum manifold.

The antibodies were diluted in casein buffer at the concentration as specified for ELISA by the manufacturer.

Samples and standards (synthetic human Aβ1-38, Aβ1-42, Aβ1-43 or Aβ1-43 peptides) were diluted in casein buffer.

Next, fluorescently labeled MTs were flowed and incubated for 10 min. Free MTs were washed with 30 µl DLB + 1 mg/ml casein buffer.

After the nitrocellulose strips were blocked with casein hydrolysate buffer (CHB) (1% casein hydrolysate in phosphate-buffered saline [PBS]/Tween) for 30 minutes at room temperature (RT), they were incubated with serum samples diluted 1 100 in CHB for 1 hour at RT.

Biotin-free casein colloidal buffer (5% casein colloid with 0.001% Merthiolate, RDI, Flanders, NJ) was used as a cassein source.

The plates were blocked with casein blocking buffer and again incubated for one hour at 37°C on an orbital table.

Filters were blocked with casein blocking buffer for 1 h at room temperature and then washed three times in double deionized water (ddH2O).

Filters were blocked with casein blocking buffer for one hour at room temperature and then washed three times in double deionized water (ddH2O).