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The Doñana case study might be considered a functional participatory approach (see Reid et al., [2009]).
The duty to contact and warn an estranged relative, as in the case study, might be seen as an example of this.
Experimental validation of another case study might be beyond the scope of the paper, but it would show a wider applicability of the method described.
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The results of this case control study might be affected by the long duration of data sampling (six semesters) and by the risk of subsequent changes in knowledge, skills and attitudes between the first and the last session.
Additionally, a positive result from a case-control study might be due to population stratification rather than linkage disequilibrium [ 49] affected by the different disease prevalence and different marker frequencies in the subpopulation thereby producing spurious associations [ 50, 51].
This is not surprising because it is likely that most of the BCIS cases in our study might be ER+ (the information on this variable is extremely sparse in BPC3) and suggests that, from a genetic point of view, ER+ and ER- tumors have different risk factors even for the first stages of carcinogenesis.
However, as we could not perform a full chromosome analysis in all cases of BL and DLBCL, some cases in the present study might be categorized into this group.
So, cases enrolled in our study might be considered as a cohort of non-HIV CD.
To determine whether the MNA cases included in our study might be biased towards a younger age range, we compared their ages at diagnosis to those of the other MNA cases in the registry and found no statistical difference (mean: 29.5 vs. 29.2 months; median: 21 vs. 24 months; p=0.968; n=16 vs. 43).
If this is the case, the results reported in this study might be explained by hypothesizing that Treg accumulate in the tumor, but fail to recognize the appropriate ligands in the context of DC to become activated to effector function.
In any case, the calculated sensitivity in this study might be underestimated due to potential overdiagnosis relevant to screen-detected cancer as mentioned above.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com