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In the case of SEC with a light scattering detector the results from the two methods are comparable.
In the case of SEC where the decision was to determine if the process drifted from a specified target then the appropriate error measure is the standard deviation.
However, sensitivity in the case of SEC was 100 times higher owing to the much bigger (about 1,000 times) sample volume used for analysis.
Also, in the case of SEC, Mori has pointed out that in aqueous SEC analyses of NaPSS the retention volume is not only governed by size-exclusion but also affected by ion-exclusion effects and hydrophobic interactions.
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In the case of Sec-dependent secreted proteins, the signal peptide is cleaved off during or shortly after the translocation [ 15- 17].
In case of the Sec translocase, some subunits (SecE, SecG and YidC) were predicted to be anammoxosomal, while some others (SecY, SecA, SecDF,YajC) were not.
Sample molar mass averages were determined by SEC using polystyrene calibration (SEC-PS) and in the case of CPUs also by SEC coupled with a multi-angle light scattering detector (SEC-MALS).
As expected, expression of both full-length and 5 kDa forms was higher in the case of Cys mutant because Sec insertion is slow in mammalian cells (Fig. 3C).
In case of UV-350, a sec-butyl group is in position six, which is a structural isomer of a tert-butyl group.
We changed the dissociation kinetic constants from 0.01 sec-1 (wild-type) to 0.1 sec-1 and then again to 0.5 sec-1 in the case of induced Tet-ON and from 5.11 × 10-4 sec-1 (wild-type) to 5.11 × 10-3 sec-1 to 2.555 × 10-2 sec-1 in the case of TetR.
Note that in both cases, Sec16 is found in large punctae reminiscent of Sec bodies.
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