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Nevertheless, our results point to a new role for LATA as a cryoprotective agent, in particular in case of oocytes.
Statistical significance was calculated using a two-tailed t-test in the Microsoft Excel 2007 program by comparing the average values obtained in sections i and iv to ii and iii in the case of oocytes, and comparing sections i and v to ii and iv for 2-cell embryos.
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The general ethical rule reflected in regulations concerning altruistic donation, namely that there must be a high chance of a good outcome for the patient, is violated in the case of oocyte donation for cloning research (George, 2007).
In the case of mouse oocytes, this method ameliorates oocyte tolerance to freezing and survival after thawing [ 35].
Cases of oocyte activation failure can currently be treated by AOA methods such as Ca2+-ionophore or strontium chloride [ 9, 133, 136, 137].
In the case of diploid oocytes with two univalent autosomes, however, 100% efficient expulsion of univalents into the first polar body would increase the frequency of lethal monosomy.
In the case of porcine oocyte-cumulus complex, both the oocyte and zona-adjacent cumulus/corona radiata cells express ZP-encoding genes [44].
The cytotoxic properties of these enzymes are probably as a consequence of their role in defense, as in the case of frog oocyte RNases [ 39], or have evolved from a defensive role, as in the case of S-RNases [ 7].
Although the treatment costs can be double or triple compared to one single hormonal stimulation for ICSI with PGD, the total costs of the accumulated cycles are lower because patients have to pay for only one ICSI procedure (in case of accumulation of oocytes) and only one genetic analysis combined with one embryo transfer.
A dysfunctional environment within the oocyte, in the case of an aged oocyte for example, is very likely to impede the process of fertilisation [ 161].
Firstly, toxicity of this cryoprotectant is brightly discussed in cases of vitrification of oocytes and embryos, when concentration is up to 20%.
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