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In the case of microorganisms, pathogen-associated molecular patterns (PAMPs) are recognized by DCs via pathogen recognition receptors (PRRs).
Whilst this assumption may be valid in the case of microorganisms growing under certain conditions, it is likely invalid in general, and especially for multicellular organisms, where cellular objectives differ greatly both between and within cell types.
Objective functions can cover a range of cellular objectives [ 3], such as maximisation / minimisation of ATP consumption, but frequently (and particularly in the case of microorganisms) take the form of an assumed "biomass" function; a hypothetical reaction that mimics cell growth rate [ 4].
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It is also suggested that in case of marine microorganisms the accumulation of large lipid droplets in the cytosol acts as an aid to buoyancy (Anderson and Wynn 2001).
An addition of aluminum to the form of 1 g/L aluminum hydroxide led to a reduction in microbial activity of 50% in the case of methanogenic microorganisms and of 72% in the case of acetogenic bacteria.
In the case of oleaginous microorganisms, some strategies are based on measurement of absorbance readings after staining with Sudan Black B (Thakur et al.1989,Patnayak and Sree 2005) or a colorimetric method based on the sulfo-phospho-vanillin reaction (Izard and Limberger 2003).
Though the microarrays have been used to great benefit in the fields of genomics and proteomics, comparatively little effort has been directed toward using cellular microarrays, particularly in the case of pathogenic microorganisms [25], [26], [27], [28], [29], [30], [31].
In the case of pathogenic microorganisms, for example, the bioactive agent may multiply after intake, substantially increasing the dose.
In the case of industrial microorganisms such as L. lactis, modification of defined regulatory networks may drastically affect the properties of the bacteria and have implications on bioprocesses.
In the case of living microorganisms, other active cellular mechanisms are involved: synthesis of specific enzymes, action of cytoplasmic or membrane proteins, and so forth [ 4, 5].
In the case of denitrifying microorganisms, the extent to which these different processes catalyze Fe(II) oxidation likely depends on the precise culturing conditions and must be evaluated on a case-by-case basis.
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