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On the contrary, Volocity does track efficiently cells on low background images such as in the case of lymphocytes detected by two photon excitation in ex-vivo lymphonodes [19], [20], [24].
In the case of lymphocytes, each experiment was performed on the cells obtained from blood of three different donors.
Contaminating normal cells were removed by immunomagnetic beads (in case of lymphocytes) or by freezing in liquid nitrogen and thawing (in case of granulocytes) (Kaspers et al, 1994b).
In case of lymphocytes stimulated with LTA, the maximum release of IL-1 β was observed at the 5th hour of reaction– 1(d)).
Such is the case of lymphocytes, neutrophils, sperm cells or epidermal cells, all of which have very limited life spans after terminal differentiation and either do not constitute solid tissues or are located close or at the open edge of a solid tissue.
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In case of lymphocyte cell, many inactive genes are present near centromeric heterochromatin (Brown et al. 1999).
In the case of T lymphocytes, these cells are selectively driven from peripheral blood to lamina propria or epithelial compartments, depending on the specific homing receptor they express.
This showed that cell death after GNP-NKCT1 treatment was significantly less in case of normal lymphocytes as compared to treated leukemic cells and also in comparison to the standard imatinib mesylate, toxicity of GNP-NKCT1 towards normal cells is less.
In the case of activated lymphocytes, it serves as an inhibitory molecule, controlling the strength and duration of the immune response [2].
As shown in Figure 5D, the assay reveals that a substantial rate of PS externalization takes place in primary mouse thymocytes without scramblase activation; as in the case of human lymphocytes and fibroblasts (Fig 5 A C), the rate was unaltered in ABCA1-deficient cells.
2D clinorotation is a well established method to simulate weightlessness on Earth [ 9, 21, 32, 46- 52] and its validity has been demonstrated through comparison with data from space experiments, e.g. in the case of T lymphocytes [ 9, 46- 51].
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