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Whole cultures were used in the case of flask fermentation and 40 ml aliquots of the homogeneous culture in bioreactor.
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As in the case of shake-flask cultivations, the medium also contained 6.7 g L−1 YNB with ammonium sulfate.
Bioreactors are systems which enable the continuous replacement of nutrients and gases either by constant agitation (as in the case of spinner flasks) or via perfusion.
Therefore, the results obtained by this method are not measured over the same time course as in the case of an Erlenmeyer flask with a culture plug.
In the case of glucose, three shake flask experiments were conducted with 0, 40 and 80 g/L glucose without changing the other medium components.
In the case of the tissue cell flasks exposed to TSV-infected hepatopancreas, ≈1.2 x 10 viral copies/μL were detected (Table).
As in the case of suspension grown in shake flasks, maximum biomass yield in CW-supplemented culture was achieved faster than in control group (Fig. 4a).
Thus, criteria to differentiate between ostrich eggshell flasks and the case of carnivore feeding are offered.
In the case of anaerobic fermentations, 250 mL shake-flasks without baffles but with a rubber stopper and a glycerol trap were used.
weight of flask + cement.
weight of flask + water.
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