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We have had no case of fixation failure up to 15 years in this relatively small series.
Since DArTs are biallelic dominant markers, PIC values range from 0 (in case of fixation of one allele) to 0.5 (when the frequencies of both alleles are equal).
In case of fixation, exclusion of dead cells was achieved by a Dead Cell discrimination kit (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany).
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In the case of fixation-related fMRI experiments, temporal jittering cannot be planned but rather results from the viewing behavior of the participants.
As described above, this result is expected, since in the case of fixations directed towards random positions the application of a correction towards the location of the stimuli will lead to an increase in the deviation from the veridical positions of the fixations.
In Group 5 there weren't any cases of fixation failure, with elongation of the graft in all the specimens.
In Group 4 there wasn't any cases of fixation failure, with graft failure in all the specimens.
This results in a low DNA yield that, in case of formalin fixation, is also partially degraded.
In case of active fixation leads the mechanism was unscrewed if possible.
In the case of PFA fixation, remaining free reactive groups were blocked by adding 50 mM NH4Cl for 10 min.
In the case of instable fixation, vessels may be imaged discontinuously and fundus structures may be distorted, which might lead to false measurements.
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