The same considerations apply in case of microbiological contamination, which is less critical in case of "cassette" systems, due to their single-use characteristics.
The overall trends in the evolution of alternative sites are the same as in the case of cassette exons.
In the case of cassette exons, we considered one of two inclusion SJs of cassette exons, having higher coverage, as the inclusion junction.
Overall, the dataset consisted of 2744 alternative splicing events, including 2126 cases of alternative splice-site usage and 618 cases of cassette exons (Additional file 1: Table S2).
In the case of a cassette carrying a β-lactamase gene encoding a novel protein sequence, the user will be referred to the β-lactamase nomenclature website (www.lahey.org/Studies/) to obtain a unique name before the new cassette is registered with RAC.
Use the same type of cassette.
In case of directly repeated cassettes the general recombination between them would lead to the deletion of part of the bacterial genome.
Consider a case of an adjacent cassette exon pair, let random variable u represent the inclusion/exclusion status of the upstream exon in a mature transcript and let d represent the status of the downstream exon.
Although C. difficile is resistant to several antibiotics [43], it has been shown that carriage of resistance genes does not necessarily confer resistance, as has been previously shown with respect to the case of the vancomycin cassette, vanGCd [44].
The 10,000-song capacity of the iPod that makes it possible to have music as a perpetual soundtrack was years away; in the Walkman era, it took a bagful of tapes and batteries to keep things going, and indecisive types would find themselves trudging around with dozens of cassettes, just in case they were overwhelmed by the need to hear, say, that John Peel Festive 50 tape.
