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As is the case for stimulation via TCR, activation of the Ras/ERK pathway by phorbol ester is preserved in TNF-treated T cells.
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This was not the case for ramp stimulation.
As was the case for hippocampal stimulation, the neurons returned to near baseline conditions following perfusion with a drug-free solution for 10 20 min (Figure 7b).
These considerations, especially in combination with the Andrews observations, seem to make the case for the stimulation hypothesis.
Contraction time was defined as the time from the onset of force production to the top of the first peak of the non-fused contraction; values for contraction time are not presented in those instances in which a distinct first peak was not discernable (which was the case for 50 Hz stimulation for the diaphragm).
Another point is that the focalization of the threshold-distance curves provided by the GS configuration is not limited to the first hundred of microns around the stimulation electrode, as it is the case for M and CB stimulations.
This however is not the case for serum or EGF stimulation [ 74].
This is also the case for P2X receptors, where stimulation associates with an intracellular acidification (Henriksen & Novak 2003).
One important consideration for a surrogate marker is the extent to which the method can be directly translated from animal to human studies, as is the case for some transcranial magnetic stimulation (Oberman et al., 2010) and MRI methodologies (van Meer and Dijkhuizen, 2010).
[Editors' note: further revisions were requested prior to acceptance, as described below. ] 1) [… In] the text of the manuscript the authors did not make a strong case for how the PT stimulation assay is being used as a proxy for protein-protein interactions.
During the acquisition phase, no significant difference was obtained between the two identical objects, except for one particular case in Acute Stimulation study with low-frequency stimulation at 400μA, and the effect was marginally significant at p=0.045.
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