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It has defined the prototype development by using win-win spiral model with use case and sequence diagram.
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In this experiment the volunteers were required to answer comprehension questions regarding a domain model specified in use cases, class, and sequence diagrams.
We report details of this case and sequencing results of the penA gene for the strain.
We collected two wild-type and two homozygous samples in each case and sequenced to 15 fold depth.
The vivax-specific PCR showed the expected bands in each case, and sequencing confirmed P. vivax A-type DNA that matched perfectly with a strain from Thailand.
SeqClean [ 37] was also used to screen all Sanger reads for vector, adaptor and E. coli genomic sequences and all 454-reads for adaptors and chloroplast sequences [ 38]; default parameters were used in this case, and sequences shorter than 100 bp were considered invalid.
Many of those tools are actively developed and possess their individual qualities, depending on the use-case and sequence data to be aligned.
YW participated in screening of the sporadic cases and sequence alignment.
The total PSNR averages for the test cases and sequences are also illustrated in Table 4.
Additionally, nested RT-PCR was conducted with ORF2-specific primer pairs (6 ) for 2 HEV isolates, 1 each from outbreak and sporadic cases, and sequences were obtained.
The consensus sequences of the donor and acceptor splice sites are AG|gtaagt and (y)nncag|GT respectively (exon terminal sequences are shown in upper case and intron sequences are given in lower case).
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