Suggestions(1)
Exact(4)
Next, we wondered if CAS resistance correlates with resistance to other cell wall injuries.
In contrast, some of the mutations identified affecting sphingolipids amount were associated to CAS resistance without affecting GS activity.
To evaluate the possible association between GS activity and enhanced CAS resistance, we determined in vitro the activity of this enzyme in the membrane fraction of the 25 mutants identified in our screening.
25 mutant strains showed enhanced CAS resistance, including 13 genes that had not been previously identified, thereby increasing significantly the number of genes associated with this phenotype (Table 3).
Similar(56)
The presence of a CRISPR spacer identical to a phage sequence adjacent to cas provides resistance against phages containing that particular sequence.
It has been reported that Cas confers resistance to anticancer drugs such as Doxorubicin and tamoxifen via activation of signaling pathways mediated by various kinases, including Src, EGFR, Akt and ERK1/2 [ 4, 5].
While Ta et al. have found that overexpression of Cas confers resistance to Doxorubicin in breast cancer cells [ 5], we found that complete lack of Cas expression also reduces cellular responsiveness to Doxorubicin, although not to the same extent than cellular responsiveness to proteasome inhibitors.
These preliminary results suggest that the role of Cas in drug resistance may be more universal, but also more complicated than previously anticipated, and suggest the intriguing possibility that gene and protein dosage may be emerging as a major mechanism to control distinct biological events in case of Cas (see Discussion).
In a world that increasingly challenges these ideals, the importance of road movies as a form of resistance can't be dismissed.
Briefly, a clean ITO substrate (surface resistance ca. 30 Ω/sq, CBC Ings Co. Ltd., Tokyo, Japan) was immersed into a solution that contains 0.25 mM HAuCl4 and 0.25 mM trisodium citrate.
The electrochemical performances of MnO2/CB/C and MnO2/CB/PVDF nanocomposites in different mass ratios are compared by areal specific capacitances (Ca), charge transfer resistance of films and retention of capacitance after 1500 cycles of charge/discharge to test stability respectively.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com