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We found that proteasome inhibition induces cell death in cells that express Cas, but not in those that lack Cas expression.
In contrast, CAS expression was the lowest in flowers.
In fact, Cas expression is considered a useful prognostic marker for patients with primary or metastatic breast cancer [ 4].
For example, in pdm null mutants, cas expression is delayed [ 3] and U5 motor neurons fail to form [ 24].
To confirm the results observed in MEFs, we used shRNAs to specifically knock-down Cas expression in human 293T and HeLa cells.
In cells with endogenous (uninduced) cas expression, the abundance of pre-crRNA and individual crRNAs was low, below 10 molecules per cell.
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To find out whether CA expression could be improved by supplementing NiCo21 DE3) cells with plasmids expressing rare tRNA, we transformed NiCo21 DE3) with pACYC-RIL, pRARE2, and pLysSRARE2.
Since estrogens and androgens are known to regulate CA expression in some tissues, we studied here whether salivary CA VI concentration shows pregnancy-related changes.
This suggests that supplementation with 4 rare codons was sufficient to improve CA expression.
We did not see a significant change in CA expression between the NiCo21 DE3) containing pACYC-RIL and pRARE2/pLysSRARE2.
Our unpublished observation indicates that E stimulates CA expression; therefore, E may indirectly stimulate intracellular HCO3− generation.
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