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Cartridges were prepared prior to the day of the experiments, followed by general methods [ 31].
The cartridges were prepared according to the manufacturer's instructions [ 20] and the settings "Research Mode, LEV ModeDNA, "DNand and "Blood/Cell" were selected for DNA extraction using the Maxwell® 16 Instrument (Promega).
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The PT-DMIP cartridge was prepared by packing the dummy molecularly imprinted polymer at the tip of the micropipette.
After centrifugation, 450 µl of substrate containing 1×MAS was added to each well, and the plate was incubated in a 37 °C non-CO2 incubator for 8 10 min. While the plate was centrifuged the XF cartridge was prepared for injections for ports A, B, C and D. Stock solution of 1 M ADP was made in water.
For gravimetric dilution series 1, 24 replicate ddPCRs were prepared from three eight-channel droplet generator cartridropletr each of the seven solutions.
The lectin columns were prepared by adding 200μl of SNA lectin to the cartridges and spun down at 1000 × g for 1 min to remove storage buffer.
For two of the serial dilution sets (gravimetric dilution series 2 and 3), eight replicate ddPCRs were prepared from a single eight-channel droplet generator cartridge for each of the seven solutions.
The four ink cartridges were filled with self-formulated enzyme or precursors solutions; the graphics files to be printed were prepared by Adobes Photoshops CC software in CMYK color space.
The inks were prepared before printing and filtered through a 0.45-μm membrane prior to filling in the color cartridges.
Validated pooled cDNA libraries were prepared for sequencing following the Illumina protocol and sequenced using the MiSeq system on a 300-cycle MiSeq sequencing cartridge (Illumina).
Buying, selling, and creating the receiver, into which a cartridge passes from the magazine and is prepared for discharge, is buying, selling, and creating a gun.
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