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A 5' truncated allele (his3-Δ5') incorporatedted into the LEU2 locus on chromosome III and substitutes a 117 bp fragment of the MAT locus carrying the recognition sequence for HO endonuclease [20] for the 5' end of the HIS3 gene.
The enzyme cleaves the arm carrying the recognition sequence, but does not cleave the arm lacking the recognition sequence.
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When objects are being carried, the recognition rate was 91.53 %.
However it was predicted that Tan1 binds tRNA and carries the recognition determinants but must function in complex with yet unidentified acetylation enzymes [ 79], reviewed in [ 105].
The key advantage of our approach is the independence of the insert DNA sequence, which can - in contrast to the conventional cloning method - internally carry the recognition sequences of the restriction enzymes used for the digestion of the target plasmid vector.
To investigate whether the DSB-induced transcriptional inhibition was specific for the MAT locus, we analyzed RNA levels and RNA polymerase II binding in tGI354 and YFP17 strains, which carried the recognition site for the HO endonuclease at the ARG5,6 locus on chromosome V or at the LEU2 locus on chromosome III, respectively.
Some unknown regulatory elements are certainly yet to be discovered, further emphasised by the down-regulation of some lipid hydrolysing genes carrying the FarA recognition site.
This protein (referred to as Ax21Xoo in the present study), however, was recently shown not to be involved in triggering an immune response in rice carrying the XA21 pattern recognition receptor (Bahar et al. 2014).
No one was ever indicted in Moore's death, and though the incident is considered by some as the start of the civil rights movement, his name doesn't carry the same recognition today as leading movement figures such as Martin Luther King Jr. and Rosa Parks.
The plasmid pCMVInt, which expresses phiC31 integrase in mammalian cells under control of the cytomegalovirus (CMV) immediate-early promoter, and the pBCPB+ plasmid, which carries the phiC31 recognition site attP and attB in direct orientation flanking a lacZ gene on a chloramphenicol resistant ColE1 derivative, were a kind gift from Dr. M.P. Calos.
The therapeutic vaccine Nexvax2 combines three proprietary peptides that elicit an immune response in patients with celiac disease who carry the immune recognition gene HLA-DQ2.
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