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We show that Y. pestis carrying the expression vector alone, the yopJ deletion mutant and the YopJ-expressing strain (Kim53ΔJ+J) all exhibit low cytotoxic potency, and preserve their virulence upon subcutaneous infection of mice (Figure 1D).
The kanamycin resistant ∆aas strain was transformed with a pUC19 vector carrying the expression construct, and after few rounds of re-streaking on BG11 agar plates containing chloramphenicol, fully segregated complementation strain ∆aas:AAE15 was obtained.
In order to assess the linearity of Gluc with respect to time, 293T cells were infected with the lentivirus vector carrying the expression cassette for Gluc.
Briefly, protein expression was induced by addition of 1 mM IPTG to a log phase culture of BL21-Gold (DE3) E. coli carrying the expression plasmid.
For real-time monitoring of ER-stress with Gluc, 293T cells infected with lentivirus vector carrying the expression cassette for Gluc.
In some experiments [5], repression was determined from the ratio of β-galactosidase activity measured for E. coli strains lacking a plasmid-borne LacR expression system to that for strains carrying the expression plasmid.
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These days, the language of Russia's official politics, and thus what shows up in state media, carries the expression of ideological purity.
Lentivirus-green fluorescent protein (LV-GFP), which carries the expression cassette for eGFP, was constructed with pLenti6 (Invitrogen).
OKF-Δ1 and OKF-Δ2 cells, where SPRR expression is downregulated to a different extent (Figure 3C), were compared to OKF control cells carrying the empty expression vector.
As a control, we used wild-type Kim53 carrying the same expression vector with the gfp gene (Kim53pGFP).
The low seed protein content cv BAL represents the genotype carrying the lowest expression for allergens.
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