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Plasmids carrying target fragment were then extracted and purified by using the Axygen mimi-prepare (Axygen), and the concentration was measured by using micro spectrophotometry (NanoDrop ND-2000, Willmington, USA).
While many aspects of adipogenesis have been validated through studies of mice carrying target mutations to key genes of adipogenesis, the role of the nutritional environment on adipogenesis during development has been neglected.
Schematic representation of recombinant TRV vectors carrying target genes.
Transformants carrying target plasmids were selected on solid GS-2 medium supplemented with erythromycin.
In this assays, we used Agrobacterium tumefaciens infiltration to co-express csi-miR164, control miRNA, GFP gene carrying target site and GFP gene carrying modified target site pair by pair.
We have used a novel flow cytometric sorting approach to specifically enrich Roche/454 DNA Capture beads carrying target DNA sequences on their surface, and reject beads carrying unspecific sequences.
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Studies of genetically engineered mice carrying targeted deletions in the genes encoding BDNF and GDNF, as well as genetic linkage analysis in humans, indicate that these trophic molecules may be candidate genes for human developmental disorders of breathing.
We [11] and others [12] have created mice carrying targeted mutations in the SirT1 gene.
To test this notion, we generated mice carrying targeted deletions of either the pro-B cell specific site HS1 or the whole cluster of DNaseI hypersensitive sites.
In addition to carrying targeting, therapeutic, and imaging motifs (pK, HSV-1 TK, and mRFP1 in this study), the flexibility of our multifunctional Ad vector platform is beneficial to the design of viral vectors to fulfill other sophisticated tasks.
These genes are critical for adipogenesis based upon correlations of their expression profiles in 3T3-L1 preadipocytes with in vitro differentiation phenotypes and later from the adiposity phenotypes found in mice carrying targeted mutations [46].
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