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We utilized an in vitro DNA repair assay and an Escherichia coli readout system with mutagenized plasmids carrying point mutations in antibiotic resistance genes.
The result showed that biotinylated XIE2008 bound wild-type p62 but not mutants carrying point mutations within ZZ domain, either D129 or C151/C154 (Fig. 3e).
Full-length p62 mutants carrying point mutations in ZZ domain that cannot interact with Nt-Arg also failed to undergo polymerization in response to Arg-Ala (Fig. 2b, top).
In this paper, the method of superposition or the Gorman method is employed to obtain an analytical solution for free and forced vibrations of cantilever plates carrying point masses.
We constructed a transgene of aPKC (aPKCAA) carrying point mutations at two predicted GSK3 consensus sites (S330A and T422A).
Sequences carrying point mutations towards the least frequent nucleotide were generated from the best DUES construct using a three-step cohesive-end PCR process.
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Among these, only two carried point mutations in gyrA leading to amino acid changes other than the phenotypically silent S95T substitution.
The method uses grid-free transport: particles which carry point values of the concentration gradient move by convection and undergo random-walk to simulate diffusion.
These mice carry point mutations in the dystrophin gene and null mutation of all utrophin genes.
mdx mice and GRMD dogs carry point mutations in the dystrophin gene.
Mutant HDV carries point mutation as indicated in the pre-S1 region of L protein.
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