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In the reciprocal experiment, a tagged version of PolgA carrying FLAG and Strep2 binding groups (PolgA.Flag.Strep2) was expressed in HEK293T cells.
To address this, two HCC cell lines, SMMC-7721 and MHCC97L, were stably infected with retrovirus carrying Flag alone or Flag-tagged Cbx4, followed by the treatment with increasing concentrations (from 0.1 to 20 μM for SMMC-7721 (Shandhai, China) and from 0.1 to 40 μM for MHCC97L (Shanghai, China)) of doxorubicin, a commonly used drug in TACE intervention.
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President Bush's team may not have examined the New York experience before introducing its own campaign advertisements with images of the ruins of the World Trade Center and firefighters carrying flag-draped remains from ground zero.
The group was carrying flags they obtained at the parade.
Hundreds of Korean fans, some of them carrying flags and banners, cheered for the visiting team.
They strolled along, most of them wearing black and carrying flags.
After the victory fans wearing Jamaica T-shirts and carrying flags flocked to greet their hero.
Peaceful!" as they marched, carrying flags and facing off with black-clad forces with helmets and riot gear.
On Sunday evening, some nationalists briefly clashed with Kurdish activists carrying flags of jailed PKK leader Abdullah Öcalan on Taksim Square.
Televised images showed boisterous opponents of the president carrying flags and blowing whistles as they celebrated today's events.
Behind me stretched a swarm of 30,000 people reeking of menthol muscle balms, some carrying flags or wearing face paint.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com