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Bacteria selected for some probiotic features (gastric juice and bile salts tolerance, high surface hydrophobicity and production of hydrogen peroxide) expressed the reporter protein GFP after transformation using a plasmid vector carrying an expression cassette assembled with lbs gene sequences.
An increased concentration of σ32 was observed in the cells carrying an expression plasmid with >200 copies of Hsh promoter.
As a second approach, parasites that already contained the pHH1-LplA1-KO plasmid were transfected with an additional plasmid carrying an expression cassette of P. berghei LplA1 ORF.
Plasmid pSL18 [31], carrying an expression cassette composed of the PSAD promoter and polyadenylation site and a paromomycin resistance cassette, was used for all subsequent manipulations.
The lentiviral vector carrying an expression cassette encoding Gluc and cerulean fluorescent protein (CFP, LV-Gluc-CFP) separated by an internal ribosomal entry site has been generated previously [2].
A corresponding negative control vector (AdTO-TK-mi) carrying an expression cassette for a negative control amiRNA instead of pTP-mi5 was also constructed.
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The mug Bobbi Brown carries into the Tuesday "heads of state" meeting carries an expression that is unprintable here.
We did not detect any GFP expression in 76 transgenic strains even though they carried an expression clone, as demonstrated by PCR analysis.
Within flight, different behaviours might also warrant differing expressions of side-bias; predator avoidance, in which the risks are much higher, or conspecific approaches, in which detailed perceptual evaluation is necessary, might carry an expression of side bias.
Ad5-KRAB-Bi-Tet-On-sTRAIL-eGFP carrying an eGFP expression cassette in the E3 region under the control of a CMV promoter was prepared as previously described [ 17].
Lewis lung carcinoma cells carrying an EGFP expression vector controlled under HRE could become a useful tool for detecting hypoxic areas in vivo when LLC cells are implanted in mice (Fig. 6).
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