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Quantitative real-time PCR was also carried on tissues extracted along the same axis.
Objective: Studies in vitro carried out on extracted teeth have demonstrated that bacterial elements may penetrate root fillings from the coronal to the apical end after a period of exposure to artificial saliva or bacterial culture.
Reverse transcription was carried out on extracted RNA using an Omniscript Reverse Transcription kit (Qiagen) according to the manufacturer's instructions, with Random Primers (Invitrogen) and RNAsin (Promega).
The profile in HPLC of crude extract was carried on a Shim-Pack CLC-ODS (H) (4.6 mm × 25 cm) was performed on a Shimadzu LC8A system, using 30% metanol/H2O acidified with 0.3% formic acid as eluent.
The present study was carried on aqueous and methanolic extracts of Aegle marmelos to investigate the presence of medicinally important phytochemicals in the leaves of different varieties/accessions.
Treatment with 0.5, 5, 50 μg/ml of Emblica officinalis extracts was carried on for 60 hours.
The weapon is carried on a special cradle inside the aircraft from which it is extracted by a parachute.
Rho-GTP pull-down assays were carried out on extracts from C2C12 cells adherent on 30 nmol/l FN for 1 hour with a GST fusion protein containing the effector domain of rhotekin (RBD-GST) as described previously [ 58].
Western blot analysis was carried out on extracts from cells transfected with control, AR, and CXCR7 siRNAs, and the expression of PSA, a model androgen-regulated gene that serves as a surrogate marker of AR transcriptional activity in prostate-cancer cells, was monitored [ 51, 52].
Toxicity tests carried out elsewhere on extracts of the plants Melia azedarach [ 28], C. myricoides [ 29] and Ajuga spp. [ 30] also showed their safety on laboratory animals at doses of 2000 mg/kg and above.
AL carried out extract preparation.
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