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However, the apparent resistance of strains normally used to carry modified genes has impeded our ability to rapidly ask basic questions about disease pathogenesis, as a 1- to 2-year backcross to DBA/1 mice is needed.
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To examine the potential conformational changes of the ACH in the presence or absence of 5'HS5 in the context of the human β-globin locus, we employed transgenic mice carrying modified human β-globin PAC transgenes (Fig. 1, [11]).
Offspring that carried modified genes were genotyped at each generation by polymerase chain reaction (PCR) amplification with specific primers for the given TG strain [34], by specific green fluorescence over the body for the KI strain and by PCR-based sequencing for the ENU mutant strain [12].
First of all, the 3' ends of mature miRNAs usually carried modified nucleotides, generated from nucleotide addition or RNA editing.
Group 2 includes so-called altered peptide ligands (APLs) carrying modified (7), mutated (8), or restricted (5) T-cell receptor-binding moieties.
In this assays, we used Agrobacterium tumefaciens infiltration to co-express csi-miR164, control miRNA, GFP gene carrying target site and GFP gene carrying modified target site pair by pair.
The development of mid oleic acid hybrids of sunflower was based on genetic stocks carrying modifying genes that limit the expression of high oleic acid content produced by mutated alleles at Ol locus (>80%) to the mid-range level (55 75%) [ 31].
The model satellite will carry a modified Michel-son-type interferometer, for studying radiation reflected from the earth's surface.
Additionally, pCS2TAL3-RRR/DDD vectors carry a modified nuclease domain that renders the homodimeric FokI an obligate heterodimer.
This study has demonstrated that serglycin and CD44 carry CS modified with disulfated disaccharides being sulfated at C4 and C6 of N-acetyl-galactosamine (CS-E units).
Hapten-positioned disulfide-connected payloads can be delivered to and into cells by bispecific cell-targeting antibodies (bsAbs) that carry a modified hapten binding entity as second specificity.
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