Among the EF2 family members, RF3 is unusual in that it does not carry domain V (Pfam name EFG_C), and instead has its own unique C terminal domain with a novel fold [ 15].
On the contrary, the N-tail loop of the same type topology seldom carries domains.
Belonging to the glycoside hydrolase family 19 (Pfam PF00182; CAZY-GH19; http://www.cazy.org), these enzymes are different from the lysozyme deployed by P2 (glycoside hydrolase family 24; PF00959) and the peptidoglycan-binding endolysin of ΦCTX (carrying domains PF01471 and PF11860).
The focus of the present study is on proteins carrying domains of the START subfamily, and we restrict the term 'START' to the subfamily of proteins that share significant sequence similarity to mammalian StAR, as described in [ 7].
Seven proteins were found to carry NRPS domains: 3 Enterobactin domains, 2 Bacitrancin domains, 1 Pyochelin domain, and 1 Cdomainmain.
One important point to note is that the various part-relationships (each of which forms a partial ordering) in the singular, plural, and mass individual domains do not carry across domains.
Hypothetical proteins that carry regulatory domains, like the FHA domain in RB1789 or a putative transcriptional regulatory domain in RB9999 are strong candidates.
Several TFs carry binding domains for various substrates or ligands, such as cNMP_binding (cyclic nucleotide-binding domain), LysR_substrate (LysR substrate binding domain), Ada_Zn_binding (metal binding domain of Ada), Fer4 (iron-sulfur cluster binding domain), PAS and GAF.
Cas proteins carry functional domains typical of nucleases, helicases, polymerases, and polynucleotide-binding proteins, involved in the propagation and functioning of CRISPR [12], [13].
If Wolbachia TCS proteins are functional, then the predicted products should carry the domains and key residues important for activity.
