Sentence examples for carries the expression from inspiring English sources
These days, the language of Russia's official politics, and thus what shows up in state media, carries the expression of ideological purity.
Lentivirus-green fluorescent protein (LV-GFP), which carries the expression cassette for eGFP, was constructed with pLenti6 (Invitrogen).
We show that Y. pestis carrying the expression vector alone, the yopJ deletion mutant and the YopJ-expressing strain (Kim53ΔJ+J) all exhibit low cytotoxic potency, and preserve their virulence upon subcutaneous infection of mice (Figure 1D).
The kanamycin resistant ∆aas strain was transformed with a pUC19 vector carrying the expression construct, and after few rounds of re-streaking on BG11 agar plates containing chloramphenicol, fully segregated complementation strain ∆aas:AAE15 was obtained.
Briefly, protein expression was induced by addition of 1 mM IPTG to a log phase culture of BL21-Gold (DE3) E. coli carrying the expression plasmid.
In order to assess the linearity of Gluc with respect to time, 293T cells were infected with the lentivirus vector carrying the expression cassette for Gluc.
For real-time monitoring of ER-stress with Gluc, 293T cells infected with lentivirus vector carrying the expression cassette for Gluc.
In some experiments [5], repression was determined from the ratio of β-galactosidase activity measured for E. coli strains lacking a plasmid-borne LacR expression system to that for strains carrying the expression plasmid.
Bacterial clones carrying the expression vector were validated using 0.5 M pyrocatechol administered drop wise on the colonies.
No enzymes were detected on the cells of strains carrying the expression plasmid lacking the anchoring signal (Additional file 3: Figure S3 and Additional file 4: Figure S4).
No extracellular enzymes were detected by Western blotting in the supernatant fluids of strains carrying the expression plasmid lacking the secretion peptide.
