Sentence examples for carries an insert from inspiring English sources

Plasmid pRW902 carries an insert with the cka promoter [ 8].

Results: Our restriction endonuclease and sequence analyses of 800 clones demonstrate that 75% of the clones carry an insert larger than 0.5 kb.

To this purpose, we used E. coli strain MC4100relA+ transformed with plasmid pBAD33 carrying an insert of each of five E. coli toxins under the regulation of the araBAD promoter.

E.coli strains MC4100 deleted with each of the five TA modules were transformed with pBAD33 carrying an insert of its cognate toxin and were grown in M9 medium containing 0.5% glycerol as a carbon source or in LB medium with chloramphenicol (50 µg/ml).

A stock that carried an insert on chromosome 3 was retained and crossed to the ubiquitous tub-GAL4 driver.

Positive transformants (clones carrying an insert of correct size) were identified by agarose gel electrophoresis as described above.

Over 85% of the BAC clones carried an insert larger than 90 kb and only 1% had inserts smaller than 50 kb.

A standard curve relating threshold cycle to DNA amount was produced by observing the number of cycles required to reach a threshold level of SYBR Green fluorescence in reactions containing known amounts of a plasmid carrying an insert that amplified with both pairs of primers.

In order to verify the random distribution of transposon insertion, we additionally investigated the relationship between the number of genes carrying an inserted transposon and the size of the corresponding genes.

All E. coli colonies potentially carrying an insert-containing vector were individually selected based on the blue/white screening.

This lean phenotype was confirmed using a second, independently derived control line carrying an insert-free vector (data not shown), thus establishing that the low fat content of gst-10-overecxpressing worms was not due to an aberrant control line.