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b) Gene/allele carriage analysis for 8 inhibitory KIR genes (2DL1, 2DL2, 2DL3, 2DL4, 2DL5, 3DL1, 3DL2 and 3DL3); 6 activating KIR genes (3DS1, 2DS1, 2DS2, 2DS3, 2DS4 and 2DS5) and two pseudo genes (2DP1 and 3DP1) will be performed for all 24 patients.
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To investigate whether being identified with an infection was associated with prior MRSA carriage, survival analysis was performed using a Cox proportional hazards model.
ODIN score, infection at ICU admission, hospitalization before ICU admission, hospital days before ICU admission, and antibiotic administration before ICU admission were identified as risk factors for imported carriage in univariable analysis (Table 1).
In addition, strains representing the prevalent genotypes of S. suis serotype 2 isolated from humans, all clustered with the pig carriage isolates on PFGE analysis.
Again when excluding non-groupable carriage isolates from the analysis, there was still a significant difference between the presence of IS1301 in the IGR of disease isolates (35%) compared to carriage isolates (26%; 49 out of 187, p<0.05).
However, the analysis of carriage duration was confounded by serotype.
Microbiological data allowed the analysis of carriage and infections due to MDR bacteria.
Variables associated with S. aureus carriage in the univariate analysis are shown in Table 3.
Noticeably, the parameter analysis of carriage duration was conducted by introducing a factor set to one as default.
Although increasing age showed a strong relationship with increasing prevalence of carriage in the univariable analysis, this increase was much reduced after adjustment for other factors.
Stool samples (up to 3) were collected from each participant for analysis of carriage of salmonellae and shigellae using standard techniques.
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