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Briefly, the library was made from a female common carp with a total of 92,160 recombinant clones and an average insert size of 141 kb.
We constructed a first generation linkage map of grass carp with a mapping panel containing two F1 families including 192 progenies.
The numbers of sequences in the paralogous pairs and gene families are shown in Table 2 and the Ks distribution plots for the two species are shown in Figure 2. We observed an obvious secondary peak in the Ks distribution of paralogous sequences within common carp (with a mode at Ks = 0.02 to 0.04), indicating a genome duplication event.
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The diets were fed to gibel carp with an initial body weight of 35.0 ± 0.2 g for 12 weeks.
One-year-old grass carp with an average weight of 180 210 g were intraperitoneally injected with 150 200 μL GCRV, a dosage of approximately 10 TCID50 kg-1 body weight.
Three-month-old grass carp with an average weight of 30 60 g were intraperitoneally injected with 50 80 μL GCRV, a dosage of approximately 10 TCID50 kg-1 body weight; fish in the control group were injected with same amount of saline.
Apparently healthy juvenile common carps, with an average initial weight of 46.25 ± 8 g were obtained from a fish farm in Shiraz, Fars province, south west of Iran.
The carp seller with a polystyrene crate on the back of his scooter cuts in front of us, turns and swears extravagantly.
The genome size of grass carp estimated with a haploid C-value of 1-1.09 1-1.091] and the conversion formulas (1 pg = 978 Mb) [ 31] wand978-1066 Mb.
Previously, we published 75,744 high-quality common carp BESs with a minimum length of 50 bp that were generated after base calling and trimming for E. coli and vector sequences [ 29].
The fish (a carp) with the highest mercury content was also analyzed for polychlorinated biphenyls (PCBs) and persistent pesticides as a probe for possible contamination with these ubiquitous pollutants within the ecosystem (Supplementary Material Section B).
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