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If no abnormal symptoms were observed, grass carp were selected for further study.
At 1d, 2d, 3d, 4d, 5d after infection ten GCRV-infected carp were selected for further study (n = 10).
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Ceruloplasmin is 4.58 times more highly expressed in resistant than in susceptible line rohu carp that were selected based on family challenge test survival to A. hydrophila[ 10].
Three infected grass carp with typical hemorrhage symptoms (infected group, n = 3) and three uninfected grass carp (healthy control group, n = 3) were selected at 5d after infection for further study.
Two pairs of grass carps with the most genetic difference were selected for artificial insemination to produce F1 offspring.
The top alignment hits were selected to calculate the difference between the common carp and zebrafish genomes at the nucleotide level.
In this study, two exposure concentrations of CFA were selected for studies into the effects of this drug in fish, specifically the carp, C. carpio.
Twenty contigs, including conserved genes, common carp specific protein-coding genes and unknown contigs [see Additional files 2: Methods S1 and 3: S2], were selected randomly.
A favorable season (24.8°C to 25.7°C) which is optimal for common carp and African catfish was selected to isolate the bacteria at maximum level.
For SNP validation, 20 adult common carp were randomly selected and blood DNA was extracted.
Therefore, Phl p 7 could be used as marker to identify multiple pollen-sensitized patients, whereas cod Gad c 1 or carp Cyp c 1 could be selected for the diagnosis of fish allergy.
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