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A total of 100 mg of pituitary tissue from summer- and winter-acclimatized carp were fixed using 4% paraformaldehyde (w/v).
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Freshly prelevated fragments of gibel carp liver were fixed in Bouin solution or 4% paraformaldehyde in PBS, dehydrated in ethanol, cleared in toluene, and embedded in paraffin.
Carps were fed to satiety once a day (09 00 a.m).
Common carp and grass carp were used as outgroups for all trees.
The grass carp were raised in clean tanks at 28°C.
Moreover, most of the type I sequences in silver carp and bighead carp are species-specific and even not fixed among the population [ 1].
Grass carp are like underwater lawn mowers.
"One thing about Carp is he's such a competitor.
But the carp are perfecting their torpedo techniques.
Around the world, carp is the No. 1 sport fish.
They work: those carp are not for catching but eating.
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