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B) The Carp sequences are generated from single pass (one strand) cDNA sequencing from RNA and, as such, are likely to contain more errors.
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In this work, carp sequences were collected and built into contigs.
Carp sequences were BLAST searched against both human and zebrafish Refseq proteins and the best hit determined the route to ortholog identification (figure 1).
B) The carp sequences were partial proteins from 6 frame nucleotide translations and from a eukaryotic and not a prokaryotic species.
The corresponding common carp sequences were extracted using their aligned coordinates and translated with the getorf program from the EMBOSS package [ 44].
The similarity searches against 4 model fish species reference genomes showed that 59.2% grass carp sequences used were considered to be significantly similar to zebrafish genome sequences.
BAC end sequences are great resources for the first genome wide survey of common carp.
Paralogs within the common carp and zebrafish sequences were identified by all-against-all BLASTN searches.
The translated carp amino acid sequences were aligned against the orthologous zebrafish protein using Clustalw [ 45].
Carp CXCL8_L2 (GenBank accession number AB470924) and zebrafish CXCL8_L1_chr1 (GenBank accession number XM_001342570) sequences were used as query in BLAST searches.
A total of 19,995 EST sequences were collected and clustered using the CAP3 [ 27] (Version Date: 04/15/05) software to create overlapping contigs of the same carp genes.
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