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Clean data were mapped to the grass carp reference genome (Bioproject: PRJNA39737, unpublished data) using TopHat2 software [ 30].
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The reference genome and annotation data of grass carp were obtained from the State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, China.
The efficiency of this approach was tested on data from rice and soybean and used to create a genetic map for common carp (Cyprinus carpio L ., which was the highest-density genetic map yet for any organism without the benefit of a reference genome sequence.
Then, mapping to human and virus reference genome were conducted.
These findings demonstrate the value of the W05 reference genome.
After sequence reads were mapped to the reference genome, mpileup files against the dog reference genome were generated using samtools48.
The cleaned reads were then aligned to the Mus musculus reference genome (Genome Reference Consortium 37, mm10) using bismark v0.16.161.
These genomes will be sequenced again and phased, as will the human reference genome, he says.
The genome described herein is the first reference genome of P. xylostella, AHIO01000000.
Using zebrafish as the reference genome, whole-genome alignments of six teleost fishes were generated.
Reads were mapped to the human genome with tophat261 to the Homo sapiens reference genome (GRCh38).
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