Sentence examples for carp cell line from inspiring English sources
The present study describes the suitability of a permanent, nonleukemic, nonvirally infected carp cell line for apoptotic studies.
A traditional approach using known apoptotic inducers such as UV-light combined with RNA interference, the latest ready-to-use technology widely used in higher vertebrates, was tested in the carp leucocyte cell line (CLC).
To determine if M-like protein SiMA promotes bacterial survival when exposed to phagocytic cells, a killing assay with the carp macrophage cell line CLC was performed.
The adherent CLC carp monocytic/macrophage cell line (European Collection of Cell Cultures no. 95070628) and the WBE27 white bass embryonic epithelial cell line (ATCC no. CRL-2773) [83] were grown at 28°C with 5% CO2.
Trout RTG-2 and carp EPC cell lines were used for virus production and titration.
As no mutations or changes in transcript level in any of the four CARP genes could be detected in the resistant R0.8 cell line, analysis of such lines may reveal additional components of that pathway in the future.
Similarly, quantitative PCR (qPCR) data comparing transcripts of each of the four CARP genes in the wild type versus the R0.8 cell line showed no difference in transcript abundance, either in the presence or absence of CpdA (see Fig. S3 in the supplemental material).
Each of the four CARP genes identified by the RNAi screen was PCR amplified from the CpdA-resistant R0.8 cell line, cloned, and sequenced for mutations in the ORF, as well as in any predicted UTR regions.
This is the first report of primary isolation of ISAV using EPC cell line, which is a non-salmonid cell line derived from a skin tumour of carp (Cyprinus carpio L). [ 29] and never before reported to be permissive to ISAV.
Like growing a cell line.
