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For this study, the possibility of activating the RNAi pathway by the use of small interfering (si RNAs was tested to inhibit in vitro viral replication of CyHV-3 in common carp brain (CCB) cells.
Virus isolation from infected fish tissues in cell culture (C. carpio carp brain and koi fin cells) was the first method to be developed (3).
CyHV-3 is widely cultivated in cell lines derived from koi fin, C. carpio carp brain, and C. carpio carp gill (3, 4, 8, 15– 17) (Table 1).
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Still the carp head north.
The abundance of 5 mRNAs were analysed in the brain of the carp under normal and challenge conditions.
According to relative studies in common carp, UCP1 mRNA levels in brain were up-regulated obviously in response to 7 10 days of cold acclimation [23].
Application of this method to isolate microsatellites from cDNA of grass carp brain got similar results (data no shown).
Furthermore, IHNV, VHSV, IPNV, KHV, NDV, AIV (H5, H7, H9), CO cell, EPC cell, FHM cell, eggs of sturgeon and salmon, tissues (muscle, pancreas, gill, spleen, fin, brain, kidney) of ornamental carps, common carps, goldfishes, and grass goldfish were used as control templates to verify the specificity of the method.
C60 is photoexcited under UV or visible light [ 42], a condition, for example, that elicited lipid peroxidation in brains of common carp (C. carpio) [ 11].
In addition, the study by Zhu and colleagues [ 41] showed that exposure to fullerene aggregates suspended in water (with average diameters of approximately 349 and/or 1,394 nm) decreased glutathione in brain of juvenile carps (Carassius auratus).
Using transcriptome sequencing, we identified 10,184 differentially expressed genes between grass carp before and after transition in brain, liver and gut.
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