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Cargo induced decreases in viability were also observed for Lipofectamine 3000 (with enhancer agent), though no significant difference between agent and agent with cargo was observed in astrocytes.
Interestingly, the autophagosomal cargo was different between CD24+ CD29hi and CD24+ CD29lo MECs; in the former (MaSC-containing) population, autophagosomes appeared largely carrying mitochondria, while in the latter (progenitor cell-containing) population, diverse cargo was observed in autophagosomes.
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Contrary to the cargo-loaded dimerization of myosin VI (Yu et al, 2009), no cargo-mediated dimerization was observed for myosin-X through the MyTH4 FERM cassette.
Excellent retention of the liposomes and the enzymatic cargo within the polymer carrier capsules was observed for up to 14 days.
Furthermore, a cargo protein of selectively autophagic degradation, p62, was observed to attach TRAF2 upon baicalin treatment.
An initial burst was observed and about ∼20 30% of cargo was released by day 4 depending upon hydrogel polymer concentration.
Interestingly, the opposite was observed for active transport in the presence of RanGTP, where net nuclear cargo accumulation was reduced for Δ15370% nuclei.
Actin staining, comparable with that of commercially available phalloidin was observed in fluorescent images of fixed cells upon cytosolic release of the cargo Lifeact.
Upon the addition of AH, which possesses a much higher binding affinity (Ka = 4.2 × 10 M–1) toward CB[7], into a cuvette containing LbL-MSNs, a soft release of the cargo molecules is observed as a result of the AH-induced dethreading of the CB[7] rings from the LbL self-assembly coatings of the MSNs.
Apparent combustion enhancement by some halon replacement fire suppressants (proposed for use in aircraft cargo bays) has been observed in full-scale, constant-volume tests at the FAA.
However, differential effects on cell-specific transgene expression and reduced viability with cargo loaded polyplex were observed.
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