Sentence examples for cargo flux from inspiring English sources
We concluded that the phosphatidylinositol 3,5-bisphosphate pathway is integral to endosome formation, determining morphology and cargo flux.
Certainly, the double photo-bleaching experiments show that the bulk of the cargo flux between Golgi is mediated by fluorescent 'dots' that function as carriers.
IFT can operate in the anterograde direction (towards the ciliary tip) using complex B factors, or in the retrograde direction (towards the ciliary base) using complex A factors; together these factors regulate the transport speed and net cargo flux.
Furthermore, some HEZs in differentiated cells were found to occur as isolated zones completely encapsulated by heterochromatin (Maul, 1977), indicative that they need not be in continuum with the ICS and maintained by high cargo flux density.
Cargo flux density and macromolecular crowding, both within the territories and the ICS, could represent additional factors maintaining such spatial arrangements (e.g., Hancock, 2004, 2007; Rippe, 2007; Richter et al, 2008; Bancaud et al, 2009).
As SQSTM1 is degraded along with its cargo, when autophagy flux is increased, its protein levels decrease; conversely, when autophagy flux is inhibited, SQSTM1 levels increase (Fig. 3B, C).
LC3-II is turned over in the lysosome along with autophagic cargo, therefore, autophagic flux can be determined by measuring the accumulation of LC3-II in response to lysosome inhibition over a short period of time.
An increased number of autophagosomes (GFP-LC3 puncta) per cell can either be the result of an increased level in autophagy initiation or of a block in later stages of autophagy when autophagosomes contact the lysosomal compartment for final cargo degradation (autophagic flux).
On the other hand, more severe injury could alter the ability of cells near the injury area to correctly deliver and degrade autophagosomal cargo, leading to inhibition of flux.
However, none of these methods are amenable to straightforward measurement of autophagy in individual cells and none of them can directly measure flux of autophagic cargo through the autophagy pathway.
Lower levels of ATP due to limited glucose supply promotes separation of these two components and causes loss of V-ATPase activity, which inhibits autophagic flux and blocks autophagy cargo degradation by lysosomal proteases [ 43– 43].
