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The cargo cell moves along the length of a Capnocytophaga cell, looping from one pole to the other.
However, the mechanism(s) of entry and the efficiency of uptake may vary with CPP identity, cargo, cell type, and other factors.
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To track endocytic cargoes, cells were washed and starved for 30 min in serum-free DMEM containing 0.5% BSA followed by 15 min incubation with labeled Transferrin (5 μg/ml) on ice.
Nucleocytoplasmic trafficking is a major consideration for the design of vehicles for the delivery of drug/DNA cargo to cell nuclei for cancer and gene therapies.
Khaki pants now have cargo and cell phone pockets.
Tp10 has been shown to transport cargo across cell membranes (3).
This is consistent with the well known ability of arginine-rich peptides to efficiently translocate cargo through cell membranes.
Their molecular cargo is cell specific [ 68], regulated by tissue physiology and cellular function, and fundamental to their bioactivity.
In order to avoid metabolic issues with the delivery system, liposomes were developed as indirect drug deposit cargo for cell function regulation.
Shorter sonication times did not result in successful transfection of the DNA cargo into cells, while prolonged sonication times affected cell viability under these test conditions.
We have shown that dendriworms are able to deliver cargo into cells efficiently at concentrations that have negligible toxic effects on cells in vitro.
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