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After treatment, cells were suspended in 2 ml mitochondria isolation buffer [0.225 M mannitol, 0.075 M sucrose, 1 mM EGTA (pH adjusted to 7.4 with 0.5 M Tris)] in a 10-ml Wheaton homogenizer tube and carefully homogenized for 30 strokes on ice.
Tissues are carefully homogenized to avoid oxygenation of the sample.
The content of the beaker was carefully homogenized and allowed to stand for 24 h.
Each mixture was carefully homogenized, moisture was adjusted to 60%% (optimal value for composting) and then the mixtures were windrowed.
The specimen was carefully homogenized with a Pasteur pipette for one minute, followed by vortexing for 30 sec–1 minute, to ensure uniform distribution of bacilli.
The samples were air dried at 30°C, powdered on a 0.5 mm mesh, carefully homogenized, and stored at −80°C in individual sterile flasks until use.
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For the preparation of the AgNP working suspension, applied to the model STP, NM-300K was diluted with ultrahigh-quality water, hand-shaken for 1 min, and sonicated for 15 min (640 W) (Bandelin, Sonorex) to disperse the AgNPs and to carefully homogenize the suspension.
The tissues were then homogenized carefully in a Precellys24 homogenizer (Bertin Corp).
The ovaries (3 g) were homogenized carefully and incubated directly in modified MRS broth.
The mixture was homogenized carefully by pipetting, resulting in ∼14 μl liposome suspension, which was then adjusted with 2 μl ATP (20 mM), 1 μl βTrCP/Skp1 (20 μM), 1 μl Cul1/Rbx1 (20 μM), and either 2 μl H2O or Neddylation reagents [1 μl APPBP1/Uba3 (5 μM), 0.5 μl UbcH12 (115 μM), 0.5 μl Nedd8 (225 μM ].
They were homogenized by carefully pipetting up and down with a 200-μl pipette tip.
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