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The bone marrow suspension was carefully agitated to obtain a single-cell suspension and centrifuged at 2000 rpm for 5 min.
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Wash cells twice with DMEM plain medium, suspend cells in blocking medium and then carefully agitate the cells at 37°C for 45 min. Reagents: Blocking buffer (PBS/heat denatured BSA).
Wash cells twice with DMEM plain medium, suspend cells in blocking medium and then carefully agitate the cells at 37°C for 45 min. Culture human bladder carcinoma ECV 304/T24 cells in cell culture medium until 100%% confluence.
After incubation for 2 3 hr at 37°C, the supernatants were carefully aspirated, 100 µL of DMSO were added to each well, and the plates agitated to dissolve the crystal product.
After incubation for 2-3 h at 37°C, the supernatants were carefully aspirated, 100 μL of DMSO were added to each well, and the plates agitated to dissolve the crystal product.
Pour the water carefully and gently to minimize any splashing, as air will become trapped in the water when it is agitated.
But social workers advising Ms. Viveiros suggested another option: continuing to have her mother carefully fed by hand, giving her only as much as she wanted and stopping if she started choking or became agitated.
Then the earthworms were removed carefully from the casting materials and transferred to a glass beaker containing 500 ml of warm (40 °C temperature) distilled water and agitated for 5 6 min with a stirrer.
"You're agitated".
They agitated.
They looked agitated.
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CEO of Professional Science Editing for Scientists @ prosciediting.com