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This staining pattern was not detected in cardiac tissue sections from dt mice processed in parallel (Fig. 1).
Furthermore, confocal microscopy demonstrated that Gpihbp1, Col18 and Lpl have a similar vascular distribution pattern in cardiac tissue sections of Col18a1−/− and wild-type mice (Fig. 6C).
As demonstrated in Fig. 6a and b, ROS generation in frozen cardiac tissue sections was examined.
Cardiac tissue sections from one of the patients were included for comparison.
A few c-Kit+tryptase− cells were observed in the human cardiac tissue sections (Fig. 2a).
To examine whether CD31+/SMA+ double-positive cells emerge after experimental MI, we analyzed cardiac tissue sections by IF.
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New microscopy tools: Wide field-of-view, high resolution image of dog cardiac tissue section taken by the Computational Imaging Lab.
Immunofluorescence on cardiac tissue (cryostat sections of 14 μm thickness) was performed as previously described [ 9, 27].
To explore if HFD led to cardiac hypertrophy, tissue sections were stained with wheat germ agglutinin FITC-labeled lectin.
Briefly, Paraffin- embedded cardiac tissue were sectioned at 5 μm, deparaffinized and then digested by proteinase K (20 μg/mL, Sigma- Aldrich, USA).
Furthermore, lovastatin also alleviated cardiotoxicity as determined by the analysis of the serum concentrations of troponin I and by monitoring cardiac fibrosis in tissue sections.
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