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In addition to lack of survival impact, CT7 positivity also had no association with organ involvement or cardiac stage.
The patient characteristics with regard to age, gender, light-chain type, cardiac stage and organ involvement are described in Table 1.
In addition, for all patients there was also an intriguing association between abnormal cIg-FISH, higher plasma cell burden and a more advanced cardiac stage at diagnosis.
Patients with abnormal FISH were more likely to have higher serum immunoglobulin FLC level (P⩽0.0001), bone marrow plasmacytosis (P⩽0.0001), NT-proBNP (P=0.009) and cardiac stage (P=0.007).
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Following targeted injections, embryos were allowed to develop to the cardiac plate stage, stage-29/30, and Nkx2.10 and TIc expression was determined.
Further studies show the generation of CiCMs passes through a cardiac progenitor stage instead of a pluripotent stage.
While there was robust ß-galactosidase reporter activity at the cardiac crescent stage, no detectable staining was seen in LS stage embryos, suggesting that initiation of Mef2cAHF enhancer expression precedes Isl1 expression and its initiation may be independent of Isl1.
BMP4 is one of the TGF-β superfamily proteins and is involved in valve development during the cardiac maturation stage.
However, the expressions of these markers in Nkx-deficient embryos were not restrained to the places where they should be confined at later cardiac differentiation stage.
A cardiac precursor stage is necessary for normal cardiomyocyte development.
Previous report also described a cardiac precursor stage in transcription factor-mediated generation of cardiomyocyte-like cells.
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