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Six hours post-irradiation, approximately 500 ul peripheral blood was collected by cardiac bleed from both irradiated and control mice.
Cerebrospinal fluid (CSF) was collected from the cisterna magna[26], and plasma was sampled via cardiac bleed from wild-type, transgenic healthy, and transgenic sick mice.
Blood plasma was collected by cardiac bleed and liver tissue collected and rapidly frozen post mortem.
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All mice were fasted for 12 h prior to any biological sampling and were euthanized by CO2 inhalation prior to end-point sampling (cardiac puncture bleed and tissue collections).
Thus, we studied this relationship including combined events, such as death, infection, cardiac arrhythmia, bleeding and pulmonary edema.
Patients with recent major surgery, wound healing difficulties, proteinuria, or significant vascular, cardiac, and/or bleeding disorders were excluded.
Prospective studies that examine cardiac and bleeding events associated with regular aspirin use among obese samples (without CVD) are needed to refine clinical guidelines in this area.
A greater number of these patients died from cardiac, cerebral, bleeding and respiratory events as compared with the patients receiving heparin.
Weekly tail bleeds and a terminal cardiac puncture bleed were collected and analyzed for TFIIIB levels via qRT-PCR.
Subsets of mice were euthanized with CO2 inhalation before terminal bleed via cardiac stick at the appropriate timepoint (0.25 96 hours).
We then performed a resuscitative thoracotomy in order to clamp the descending aorta and perform direct cardiac massage because we expected that the cause of cardiac arrest was bleeding from the severe pelvic fracture.
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